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The systematic breeding method was adopted to breed a new good cultivar of Curcuma longa, named "Chuanjianghuang 1". From 2014 to 2015, two consecutive years of multi-point test were carried out in Shuangliu, Chongzhou and Wenjiang. The biological characters, phenology, agronomic characters, yield and quality indexes of "Chuanjianghuang 1" were comprehensively evaluated. The results showed that compared with local traditional species, the rhizome yield of the new cultivar "Chuanjianghuang 1" increased by 20.61%.The average content of volatile oil was higher than 24.17% and the average content of curcumin in root tuber was higher than 26.62%. The yield of root tuber increased by 54.59%.The average content of volatile oil is higher than 36.28% and the average content of curcuminoids is higher than 25.31%. Compared with "Huangsi Yujin 1", "Chuanjianghuang 1" increased the average yield of rhizome by 123.68%,the volatile oil increased by an average of 7.69%and the curcumin content increased by an average of 58.23%. The average content of volatile oil is higher than 52.82% and the average content of curcuminoids in root tuber was higher than 38.34%. The new variety "Chuanjianghuang 1" has better yield than the local traditional species, and the internal quality of rhizome and root tuber is better. Compared with "Huangsi Yujin 1", the yield of rhizome is significantly increased, and the internal quality of rhizome and root tuber is better, especially the content of curcumin in rhizome and curcuminoids in root tuber is significantly higher than that of "Huangsi Yujin 1". "Chuanjianghuang 1" is high yield, good quality, good stability and strong adaptability, which is suitable for cultivation and promotion in Chengdu Jinma River Basin, such as Shuangliu, Chongzhou, Wenjiang.
Subject(s)
Breeding , Curcuma , Diarylheptanoids , Oils, Volatile , RhizomeABSTRACT
OBJECTIVE: To analyse the clinical value of uterine artery embolization(UAE)in the treatment of caesarean scar pregnancy(CSP)in the second trimester of pregnancy.METHODS: The clinical data of 29 cases treated from January2013 to June 2018 in Shengjing Hospital of China Medical University was retrospective analyzed.The patients were divided into group A(14 cases)and group B(15 cases)according to the first hospital. visitedPatients in group A first visited basic local hospitals and underwent drug-induced labor.Due to a large amount of vaginal bleeding or failure of induced labor,they were transferred to our hospital for further treatment. Patients in group B first visited our hospital and were treated by UAE before induced labor. The therapeutic effect was compared between the two groups.RESULTS: There was no statistical difference between the two groups in age,gestational weeks,number of caesarean section,period of time from previous caesarean section,or number of previous induced abortions(P>0.05).The average operation time of group B was shorter than that of group A[(54.00±31.19)min vs.(105.07±67.92)min,P<0.05]. The average intraoperative bleeding of group B was less than that of group A[(73.33±24.57)mL vs.(869.29±276.99)mL,P<0.05].Postoperative vaginal bleeding time of group B was shorter than that of group A[(4.46±1.06)d vs.(6.79±2.08)d,P<0.05].Average hospitalization time of group B was shorter than that of group A[(8.80±1.74)d vs.(14.57±4.54)d,P<0.05].The transfusion rate of group B was lower than that of group A[0 vs.78.6%,P<0.05].The uterine retention rate of group B was higher than group A[100% vs.71.4%,P<0.05].CONCLUSION: UAE has significant clinical value in the treatment of CSP in the second trimester of pregnancy.It can reduce operation time,intraoperative bleeding volume and increase uterine retention rate.
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OBJECTIVE: To investigate the inhibitory activity, induced differentiation-inducing activity and apoptosis-inducingactivity of hydroxyl morpholine (QDML-01) on chronic myelocytic leukemia cells line K562. METHODS: The cell growth curve was drawn based on cell counting method. The IC50 value of QDML-01 and positive control medicine to K562 cells were evaluated by methyl thiazolyl tetrazolium (MTT) assay method. Double soft agar assay method was carried out to study the ability of cell proliferation to determine efficacy of phamacognosy. The pathomorphism was analyed by the Wright-Giemsa staining method. The mechanism of cell apoptosis from morphology and gene level were investigated, by AO-EB double-staining method and DNA breakage test. The effect of QDML-01 on K562 cells from the protein level was determined by Western-blot. RESULTS: The growth curves showed the K562 cells had strong cell vitality. They came into logarithmic phase on the third generation. The MTT assay results showed that the IC50 values of QDML-01 and imatinib to K562 cells were 5. 81 and 596.88 nmol ·L-1. Double soft agar colony formation test showed that clone formed at 21 d and the inhibitory rate of QDML-01 was 81.7%. It indicated that K562 cells were sensitive to QDML-01. Morphology test result showed that QDML-01 induced K562 cells to normal cells. The results of AO-EB double-staining method showed that QDML-01 induced the apoptosis of K562 cells. The study of DNA breakage test indicated that QDML-01 can induce the apoptosis of K562 cells to produce DNA banding with step-like. Western-blot analysis result suggested that QDML-01 can downregulated the expression of P210bcr/abl protein. CONCLUSION: QDML-01 has the inhibitory activity on chronic myelocytic leukemia cells line K562 by promoting the apoptosis of K562 cells and inducing differentiation to normal cells.
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The relationship of interleukin-4 (IL-4) C-33T and C-590T (C-589T) gene polymorphisms with allergic rhinitis was analyzed. Data about the case control studies of IL-4 gene promoter polymorphisms [C-33T and C-590T (C-589T)] and their association with allergic diseases and correlation between serum IL-4 levels and allergic rhinitis were retrieved. The Stata 12.0 statistical software was applied to analyze the correlation between IL-4 gene polymorphisms and allergic rhinitis. The meta-analysis result of TT/CC genotype of -590 (-589) polymorphism showed a significant association with allergic diseases [OR=1.93, 95% CI (1.61-2.31), P=0.00]. Meta-analysis of the TT+TC versus CC genotype of IL-4 C-33/T polymorphism revealed significant associations with allergic diseases [OR=3.23, 95% CI (1.13-9.25), P=0.03]. Meanwhile, there was a significant correlation between serum IL-4 levels and allergic rhinitis [OR=2.52, 95% CI=(1.80-3.23), P=0.00]. IL-4 gene -590 TT genotype may increase the risk of allergic rhinitis and the T allele mutation of -33 might be correlated with allergic rhinitis.
Subject(s)
Humans , Gene Frequency , Genotype , Interleukin-4 , Blood , Genetics , Polymorphism, Single Nucleotide , Promoter Regions, Genetic , Genetics , Rhinitis, Allergic , Rhinitis, Allergic, Perennial , Blood , Genetics , Risk FactorsABSTRACT
The relationship of interleukin-4 (IL-4) C-33T and C-590T (C-589T) gene polymorphisms with allergic rhinitis was analyzed. Data about the case control studies of IL-4 gene promoter polymorphisms [C-33T and C-590T (C-589T)] and their association with allergic diseases and correlation between serum IL-4 levels and allergic rhinitis were retrieved. The Stata 12.0 statistical software was applied to analyze the correlation between IL-4 gene polymorphisms and allergic rhinitis. The meta-analysis result of TT/CC genotype of -590 (-589) polymorphism showed a significant association with allergic diseases [OR=1.93, 95% CI (1.61-2.31), P=0.00]. Meta-analysis of the TT+TC versus CC genotype of IL-4 C-33/T polymorphism revealed significant associations with allergic diseases [OR=3.23, 95% CI (1.13-9.25), P=0.03]. Meanwhile, there was a significant correlation between serum IL-4 levels and allergic rhinitis [OR=2.52, 95% CI=(1.80-3.23), P=0.00]. IL-4 gene -590 TT genotype may increase the risk of allergic rhinitis and the T allele mutation of -33 might be correlated with allergic rhinitis.
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<p><b>BACKGROUND</b>There are definite gender differences in patients with macular holes. Menopausal women over 50 years are most affected. We aimed to observe the effect of estrogen on collagen gel contraction by cultured human retinal glial cells. It is speculated that estrogen could strengthen the tensile stress of the macula by maintaining the correct morphology and contraction.</p><p><b>METHODS</b>Estrogen was used to determine its effects on collagen gel contraction, and its function was measured using morphological changes in cells. Human retinal glial cells were cultured in collagen solution. The cells were then exposed to collagen gels and the degree of contraction of the gel was determined.</p><p><b>RESULTS</b>Estrogen at differing concentrations had no effect on the growth of human retinal glial cells. However, after exposed to collagen gel block, less contraction was noted in the estrogen-treated group than in the control group.</p><p><b>CONCLUSIONS</b>Estrogen can inhibit collagen gel contraction by glial cells. These results suggest a mechanism for macular hole formation, which is observed in menopausal females.</p>
Subject(s)
Female , Humans , Cells, Cultured , Collagen , Metabolism , Enzyme-Linked Immunosorbent Assay , Estrogens , Pharmacology , Neuroglia , MetabolismABSTRACT
<p><b>OBJECTIVE</b>To observe the clinical efficacy of electroacupuncture (EA) on mild cognitive impairment (MCI) for patients of Uygur and Han nationality and explore the national diversity among the patients with MCI.</p><p><b>METHODS</b>Twenty-five cases were divided into Han nationality group (15 cases) and Uygur nationality group (10 cases) according to patient's nationality. In either group, EA was applied to Baihui (GV 20), Fengchi (GB 20), Xuanzhong (GB 39), Fuliu (KI 7), Sanyinjiao (SP 6) and Taixi (KI 3), once per day, 15 treatments made one session and there were 5 days at the interval among the sessions. Totally, 3 sessions of treatment were required. The proton magnetic resonance spectroscopy (1H-MRS) was used to observe the changes in the ratio of N-acetylaspartate and creatine (NAA/Cr) on the left hippocampus for the patients in two groups before and after treatment as well as the changes in the results of the Mini-Mental State Examination (MMSE) and the Montreal Cognitive Assessment (MoCA) separately.</p><p><b>RESULTS</b>NAA/Cr in Uygur nationality group was higher than that in Han nationality group before treatment (1.659 +/- 0.418 vs 1.137 +/- 0.190, P < 0.05). After treatment, MMSE and MoCA scores all increased apparently as compared with those before treatment in two groups (P < 0.05, P < 0.01), and NAA/Cr on the left hippocampus in either group was up-regulated as compared with that before treatment (both P < 0.01).</p><p><b>CONCLUSION</b>EA can improve the overall cognitive function for the patients with MCI. There is the national diversity in the partial brain metabolite level between Uygur patients and Han patients with MCI.</p>
Subject(s)
Aged , Aged, 80 and over , Female , Humans , Male , Middle Aged , Aspartic Acid , China , Ethnology , Cognition Disorders , Metabolism , Therapeutics , Creatine , Electroacupuncture , Hippocampus , Chemistry , Magnetic Resonance SpectroscopyABSTRACT
This paper introduce the professor WU Lian-zhong's clinical experience in acupuncture treatment for facial paralysis. Professor WU emphasizes the treatment based on syndrome differentiation. The appropriate theory, method, prescription and acupoints should be applied according to observing the subtle details of syndromes and differentiating the state of diseases development, so as to obtain effectiveness, to shorten the duration of treatment course and to reduce sequelae. The concrete methods of prognosis diagnosis for facial paralysis and three effective cases are introduced.
Subject(s)
Adult , Aged , Child , Female , Humans , Male , Acupuncture Therapy , Facial Paralysis , TherapeuticsABSTRACT
<p><b>OBJECTIVE</b>To probe into effectiveness and safety of acupoint sticking therapy for simple obesity.</p><p><b>METHODS</b>One hundred and eight cases were randomly divided into an acupoint sticking group (n = 58) and an acupuncture group (n = 50). The acupoint sticking group were treated with TCM plaster sticking at Zhongwan (CV 12), Guanyuan (CV 4), Qihai (CV 6), etc. The acupuncture group were treated with simple acupuncture at Zhongji (CV 3), Guanyuan (CV 4), Qihai (CV 6), etc. Their therapeutic effects were observed after 3 therapeutic courses.</p><p><b>RESULTS</b>The total effective rate was 84.5% in the acupoint sticking group, 86.0% in the acupuncture group with no significant difference between the two groups (P > 0.05); after treatment, the body weight, body mass index, waist circumference, hip circumference decreased and the cumulative score of clinical symptoms decreased in the two groups with no significant difference between the two groups, but the waist-hip ratio did not significantly change.</p><p><b>CONCLUSION</b>TCM acupoint sticking therapy has a definite therapeutic effect on simple obesity.</p>
Subject(s)
Adult , Female , Humans , Male , Middle Aged , Acupuncture Points , Acupuncture Therapy , Methods , Medicine, Chinese Traditional , Obesity , TherapeuticsABSTRACT
<p><b>OBJECTIVE</b>To test the therapeutic effect, safety of acupoint application for treatment of constipation.</p><p><b>METHODS</b>Forty-two cases were randomly divided into a treatment group of 22 cases and a control group of 20 cases. The treatment group were treated with acupoint application, with the cake made by Sanleng (Rhizoma Spargani), Ezhu (Rhizoma Zedoariae), Dahuang (Radix et Rhizoma Rhei) and Bingpian (Borneolum), which was applied at Tianshu (ST 25), Qihai (CV 6), Guanyuan (CV 4); the control group were treated with oral administration of Congrong Tongbian Oral Liquid.</p><p><b>RESULTS</b>The total effective rate was 81.8% in the treatment group and 50.0% in the control group, the treatment group being better than the control group (P < 0.05); the first defecation time was (5.1 +/- 2.8) h in the treatment group and (10.1 +/- 7.3) h in the control group, with a significant difference between the two groups (P < 0.05).</p><p><b>CONCLUSION</b>TCM acupoint application therapy has a definite therapeutic effect on constipation.</p>
Subject(s)
Adult , Aged , Female , Humans , Male , Middle Aged , Acupuncture Points , Constipation , Therapeutics , Medicine, Chinese TraditionalABSTRACT
Professor WU Lian-zhong not only has meticulous scholarship, great learning, rich clinical experience, obvious therapeutic effects, but also is good at exploration and writing, weeds through the old to bring forth the new. The present paper introduces that the authors apply "Wuxinxue" association summarized by professor WU to treat various kinds of intractable insomnia and achieves very good results, which is a convenient and unique method, and 5 successful samples are given.
Subject(s)
Adult , Aged , Female , Humans , Male , Middle Aged , Acupuncture Points , Acupuncture Therapy , Methods , Medicine, Chinese Traditional , Sleep Initiation and Maintenance Disorders , TherapeuticsABSTRACT
<p><b>OBJECTIVE</b>To summarize advances of studies on treatment of dysmenorrhea with acupoint application in recent 10 years so as to provide references and basis for scientific research and clinical works in future.</p><p><b>METHODS</b>The literature about treatment of dysmenorrhea with acupoint application in the recent 10 years are reviewed from treatment based on syndrome types, acupoint selection, selection of excipients, application time, assessment of therapeutic effects, adverse effects and other aspects.</p><p><b>CONCLUSION</b>Acupoint application has a definite therapeutic effect on dysmenorrhea, but with some problems in clinical research.</p>
Subject(s)
Female , Humans , Acupuncture Points , Acupuncture Therapy , Biomedical Research , Dysmenorrhea , Therapeutics , SyndromeABSTRACT
<p><b>OBJECTIVE</b>To Sum up advances of studies on acupuncture for treatment of obesity in recent 10 years and to put forward the problems should be paid attention to and the development direction in clinical scientific research.</p><p><b>METHODS</b>Review the literature about acupuncture and moxibustion slimming therapy in recent 10 years from characteristics of acupuncture and moxibustion in selection of acupoints, analysis of various therapeutic methods, problems in clinical research.</p><p><b>CONCLUSION</b>Acupuncture and moxibustion treatment of obesity has obvious therapeutic effect and less side-effects with flexible selection of acupoints and more methods.</p>